FIGURE 2.

Importance of Ser²⁵⁸ for copper homeostasis in vivo. Growth curves of yeast expressing Ccc2 wt (circles), S258A (squares), and D627A (triangles) at 30 °C in two different media. Left, growth curves in iron and copper-limited medium (open symbols). The inset represents A₆₀₀ at 38 h for Ccc2 wt and S258A yeast cell cultures (as indicated) after correction for the non-functional D627A mutant. Right, yeast growth in copper-supplemented medium (closed symbols). Composition of the media is described under “Experimental Procedures.” Data are mean ± S.E. of three separate experiments.