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. 2010 Dec 14;286(9):6911–6917. doi: 10.1074/jbc.M110.194662

FIGURE 4.

FIGURE 4.

Different membrane-anchoring modalities do not affect PS-DNA-induced PrP down-regulation. a, Western blot analysis of N2a cells transiently transfected with the indicated plasmid constructs. PrP-CD4, fusion of PrP aa 1–230 and the transmembrane domain of mouse CD4 (aa 369–431); PrP-Thy1, fusion of PrP (aa 1–231) and the GPI anchor of Thy1 (aa 127–163); NT-Thy1, fusion of aa 1–22 of PrP, the FLAG epitope, PrP N terminus (aa 23–88), and Thy1. Thy1 was detected using anti-HA antibodies, and NT-Thy1 was detected using anti-FLAG antibodies. All other PrP forms were detected using the 3F4 monoclonal antibody. MW, molecular mass markers. b, densitometric quantification of Western blot analyses (n = three independent studies, p = 0.0001, p = 0.005, p = 0.0004 for 3F4PrP, PrP-Thy1, and for NT-Thy1, respectively).