FIGURE 6.

Attachment of PrP at the C terminus of the cytosolic proteins YFP and CFP promotes PS-DNA susceptibility. a, Western blot analysis of CHO cells transiently transfected with CFP-PrP or YFP-PrP fusion products and treated with PS-DNA. (n = two independent studies). MW, molecular mass markers. b, densitometric quantification of Western blot analyses in panel a. c, Western blot analysis of N2a cells transiently transfected with CFP-PrP or YFP-PrP fusion products and treated with PS-DNA. d, positions of YFP or CFP insertion in PrP. SS, signal sequence; PO, pre-octarepeat domain; OR, octarepeat domain; GPI, glycosylphosphatidylinositol anchor.