FIGURE 5.

ClustalW sequence alignment of cucumber AORs with nearest sequences of various plants. The sequence is shown in single-letter amino acid code. Double arrows show peptide sequences used for designing degenerate primers according to the 5′- and 3′-RACE cloning method. Dotted arrow indicates a variable peptide sequence region. An NADPH-binding Rossmann-like domain is indicated by a box. The asterisks below the sequence indicate the positions of fully conserved residues. Accession numbers are BAJ23910 (cucumber chloroplastic), BAJ23911 (cucumber cytosolic), ACU19769 (soybean), AAK66565 (sunflower), BAF23605 (rice), ACG41966 (maize), and AAM16188 (Arabidopsis).