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Kinetic parameters of recombinant AtAOR
| Chain length | kcat | Km | kcat/Km | |
|---|---|---|---|---|
| s−1 | mm | s−1mm−1 | ||
| Acrolein (propenal) | 3 | 22 ± 4.7 | 3.5 ± 0.4 | 6.28 ± 1.5 |
| Crotonaldehyde (butenal) | 4 | 1.3 ± 0.2 | 1.8 ± 0.2 | 0.72 ± 0.14 |
| 3-Buten-2-one | 4 | 3.5 ± 0.4 | 0.13 ± 0.02 | 26.9 ± 5.2 |
| 1-Penten-3-one | 5 | 4.0 ± 0.4 | 0.29 ± 0.03 | 13.8 ± 2.0 |
| 4-Hexen-3-one | 6 | 3.3 ± 0.3 | 0.12 ± 0.02 | 27.5 ± 5.2 |
| NADPHa | 23 ± 4.8 | 0.01 ± 0.001 | 2300 ± 530 | |
| NADHa | 23 ± 4 | 0.053 ± 0.006 | 434 ± 90 |
a Acrolein (20 mm) was used as a substrate.
