FIGURE 2.
The increase in FBLN5 expression elicited by hypoxia is mediated by the PI3K/Akt/mTOR pathway. BAEC were preincubated with rapamycin (Rap; 100 nm) or LY294002 (LY; 10 μm) and exposed to normoxia (Norm, controls) or hypoxia (1% O2, 24 h). A, HIF-1α protein levels were analyzed by Western blot. β-Actin levels were used as a loading control. A representative autoradiogram of three independent experiments performed by duplicate is shown. B, FBLN5 mRNA levels were evaluated by real-time PCR. Data are expressed as mean ± S.E. (n = 9; * and †, p < 0.05 versus control cells and versus hypoxic cells, respectively).