FIGURE 4.

HIF-2α silencing does not alter hypoxia-induced FBLN5 up-regulation. A, HIF-1α and HIF-2α mRNA levels, determined by real-time PCR, were analyzed in HUVEC transfected with a HIF-2α-specific siRNA (siHIF-2α) or a control siRNA (siRAND). Data are expressed as mean ± S.E. (n = 6; *, p < 0.05 versus siRAND cells). HIF-2α knockdown did not affect the up-regulation of FBLN5 mRNA (B) and protein levels (C) triggered by hypoxia (Norm, normoxia; Hyp, hypoxia). Results are expressed as mean ± S.E. (n = 6; *, p < 0.05 versus normoxic cells transfected with a siRAND).