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. 2010 Dec 23;286(9):7149–7160. doi: 10.1074/jbc.M110.175190

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Analysis of Zn1, Zn2, and Zn3 binding to ssDNA and ssRNA probes. A, binding assays were carried out with ³²P-labeled poly(dA), poly(T), or poly(dC) (30 pmol) and Zn1, Zn2, or Zn3. Retarded bands are indicated by arrowheads. N, no protein. B, instead of ssDNA probes, ³²P-labeled poly(rA), poly(U), and poly(rC) (30 pmol) were used. C, biotinylated poly(dC) or poly(U) (0.25 pmol) and ³²P-labeled poly(rA), poly(U), or poly(dC) (0.25 pmol) were used. After incubation of a biotinylated and a ³²P-labeled oligonucleic acid with PARP-1, Zn3, or the DBD domain, biotinylated oligonucleic acid was pulled down by avidin-Sepharose. ³²P-labeled oligonucleic acid was analyzed using urea-10% polyacrylamide gels.