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. 2011 Jan 5;286(9):7214–7226. doi: 10.1074/jbc.M110.169342

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — A, S100B induces shape changes in BV-2 microglia in a RAGE-dependent manner. BV-2/mock, BV-2/RAGEΔcyto, and BV-2/RAGE microglia were cultivated on glass coverslips, treated for 3 h with increasing concentrations of S100B, and fixed. The cells were subjected to immunofluorescence using a monoclonal anti-tubulin antibody (green) and then treated with rhodamine-phalloidin to stain F-actin (red). The nuclei were counterstained with DAPI (blue). Shown is one representative field for each condition. A quantitative analysis is also shown. B, schematic representation of the molecular mechanism whereby S100B stimulates microglia migration via RAGE engagement. Through multiple pathways S100B/RAGE stimulates the expression (arrow) and release of chemokines that in turn chemoattract microglia. In addition, S100B activates RAGE/diaphanous-1/Ras/PI3K/RhoA/ROCK and RAGE/diaphanous-1/Cdc42-Rac1 pathways that cause the cytoskeleton rearrangement and cell shape changes required for microglia motility.