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Expression of VKOR and the carboxylase in coinfected cells. Duplicate samples of intact washed microsomes from insect cells coinfected with baculoviruses containing the carboxylase and either wild type or mutant VKORs were quantitated by LiCor analysis on Western blots that detected VKOR using an antibody against the C terminus of VKOR (a) and carboxylase using an antibody against the C-terminal FLAG epitope (b).
