FIGURE 4.

Comparison of the activities of TA domains of NFI-X1 and NFI-X3. Primary human astrocytes and HEK cells were transiently transfected with 100 ng of p5xGAL4-Luc, 400 ng of the indicated expression plasmids, and 50 ng of β-galactosidase expression vector. One day after transfection, cells were harvested. (B) Luc activities were normalized to β-galactosidase activities to account for transfection efficiency. (A) The inset shows the expression levels of fusion proteins. Experiments were performed three times in duplicate. DBD, DNA-binding domain. Error bars, S.D.