FIGURE 4.

Determination of the catalytic kinetics of Atg4C and Atg4D. A and B, different concentrations of LC3B-GST and GATE-16-GST as indicated were incubated with 0.025 mg/ml Atg4C (A) or Atg4D (B) in a volume of 20 μl for 5 h. The reactions were stopped, and the mixture was resolved by SDS-PAGE and CBB staining. C, the initial velocity of the reaction at each substrate concentration was plotted, and the kinetics was determined as described in the legend to Fig. 2D.