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The impact of the N-terminal sequences on the catalytic efficiency of Atg4B. A, different concentrations of LC3B-GST or GATE-16-GST were incubated with Atg4BΔ1–24 (5 μg/ml) in a volume of 20 μl for 30 s. The reactions were stopped and resolved by SDS-PAGE and CBB staining. B, the initial velocity of the reaction at each substrate concentration was plotted, and the kinetics was determined as described in the legend to Fig. 2D.
