TABLE 1.
LC3B-GST | GATE-16-GST | GABARAP-GST | Atg8L-GST | |
---|---|---|---|---|
Km (10−5 mol/liter) | ||||
Atg4A | 3.39 ± 0.51 | 1.57 ± 0.46 | 2.08 ± 0.75 | 3.67 ± 0.30 |
Atg4B | 0.51 ± 0.05 | 0.61 ± 0.07 | 0.58 ± 0.05 | 0.44 ± 0.23 |
Atg4C | 4.75 ± 0.24 | 1.24 ± 0.13 | ND | ND |
Atg4D | 1.31 ± 0.27 | 0.72 ± 0.36 | ND | ND |
Atg4BC74S | 0.68 ± 0.04 | 0.44 ± 0.08 | 0.84 ± 0.09 | 0.35 ± 0.06 |
Atg4BΔ1−24 | 0.59 ± 0.06 | 0.94 ± 0.13 | ND | ND |
kcat/Km (mol−1 liter s−1) | ||||
Atg4A | 58.5 | 12,800 | 2,460 | 1,960 |
Atg4B | 89,600 | 107,000 | 78,900 | 88.800 |
Atg4C | 12.2 | 20.3 | ND | ND |
Atg4D | 34.2 | 32.8 | ND | ND |
Atg4BC74S | 43.6 | 65.9 | 10.9 | 16.5 |
Atg4BΔ1−24 | 118,000 | 121,000 | ND | ND |