FIGURE 1.

Effect of doxorubicin on induction of cell death in U-937, HeLa, THP1, SKBr3, and MCF-7 cells. A, THP1, U-937, HeLa, MDA-MB-231, and MCF-7 cells were treated with 1 μm doxorubicin for different times, and inhibition of cell viability was calculated from the MTT assay. B, U-937, THP1, MCF-7, HeLa, and SKBr3 cells were treated with 1 μm doxorubicin for 72 h. Cells were fixed, stained with propidium iodide, and viewed under a fluorescence microscope. U-937, THP1, HeLa, and MCF-7 cells were treated with doxorubicin (1 μm) for 72 h. Cells were incubated with LIVE/DEAD assay solution for 30 min. Cells were viewed under a fluorescence microscope. C, the number of apoptotic cells (red color) and live cells (green color) was counted, and the percentage of apoptotic cells is indicated in the figure. MCF-7 and HeLa cells were treated with doxorubicin (1 and 2.5 μm) for 24 h. Cells were harvested, and DNA was prepared and run in a 1.5% agarose gel. D, the gel was stained with ethidium bromide and viewed in Gel Doc.