Skip to main content
. 2010 Dec 14;286(9):7339–7347. doi: 10.1074/jbc.M110.191916

FIGURE 2.

FIGURE 2.

Effect of doxorubicin on induction of p53 and cell death in p53-positive and -negative cells. A, MCF-7 and SKBr3 cells were treated with different concentrations of doxorubicin for 24 h, and nuclear extracts were assayed for p53 DNA binding by gel shift assay. HCT116 cells (HCT116 (Wild)) and p53 knock-out cells (HCT116 (p53−/−)) were treated with 1 μm doxorubicin for different times. B, upper panels, NE were prepared, and 8 μg of NE proteins per sample was used to detect p53 and NF-κB by gel shift assay. Lower panels, whole-cell extracts were prepared, and 100 μg of proteins was used to detect p53 and p21 by Western blot. Blots were reprobed for tubulin. HCT116 p53+/+ and wild type cells were treated with 1 μm doxorubicin for different times, and cell viability was detected by MTT assay. C, inhibition of cell viability was indicated in the figure.