TABLE 1.

Primers used for mutation or deletion constructs of ATP7B

Primer name	Sequence
D1027N forward	5′-CAAGATAAAGACTGTGATGTTTAACAAGACTGGCACCATTACCC-3′
D1027N reverse	5′-GGGTAATGGTGCCAGTCTTGTTAAACATCACAGTCTTTATCTTG-3′
C983A and C985A forward	5′-TGCTGTGCATTGCCGCCCCCGCCTCCCTGGGGCTGG-3′
C983A and C985A reverse	5′-CCAGCCCCAGGGAGGCGGGGGCGGCAATGCACAGCA-3′
C575A and C578A forward	5′-CAATCACAGGGATGACCGCCGCGTCCGCTGTCCACAACATAGAGT-3′
C575A and C578A reverse	5′-ACTCTATGTTGTGGACAGCGGACGCGGCGGTCATCCCTGTGATTG-3′
S478/481A forward	5′-GGACATCTTGGCAAAGGCCCCACAAGCAACCAGAGCAGTGGCAC-3′
S478/481A reverse	5′-GTGCCACTGCTCTGGTTGCTTGTGGGGCCTTTGCCAAGATGTCC-3′
S1121A forward	5′-CACAGTGAGCGCCCTTTGGCTGCACCGGCCAGTCACCTGA-3′
S1121A reverse	5′-TCAGGTGACTGGCCGGTGCAGCCAAAGGGCGCTCACTGTG-3′
S1453A forward	5′-ACGATGATGGGGACAAGTGGGCTCTGCTCCTGAATGGCAGGG-3′
S1453A reverse	5′-CCCTGCCATTCAGGAGCAGAGCCCACTTGTCCCCATCATCGT-3′
Δ5 forward	5′-GTCAGGATCTTGGGCATGACTGTGTCTAACATAGAAAG-3′
Δ5 reverse	5′-CTTTCTATGTTAGACACAGTCATGCCCAAGATCCTGAC-3′