TABLE 1.
S. cerevisiae strains used in this study
Strain | Genotype and plasmid | Origin |
---|---|---|
BY4741 | MATa, his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Euroscarf |
BY4742 | MATα, his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | Euroscarf |
Y05574 | as BY4741, kre6Δ::kanMX4 | Euroscarf |
Y04773 | as BY4741, skn1Δ::kanMX4 | Euroscarf |
YKY213 | as BY4741, ura3-52 | This study |
YKY276 | as BY4742, ura3-52 | This study |
SAY181 | MATa his3Δ leu2Δ trp1Δ ura3Δ ypt11Δ::LEU2 OCH1-3HA::HIS3 pRS73 (YPT11 TRP1 CEN) | Arai et al. (28) |
KSY466 | as YKY276, ura3-52::(YPT1promoter-GFP-LIP1 URA3) | This study |
YKY218 | as YKY213, ura3-52::(TPIpromoter-GFP-SNC1 URA3) | This study |
KTY393 | as YKY276, ura3-52::(YPT1promoter-GFP-KEG1 URA3) | This study |
KTY634 | as YKY213, ura3-52::(YPT1promoter-GFP-KEG1 URA3), keg1Δ::kanMX4 | This study |
KTY284 | as BY4741, KRE6-3HA LEU2 | This study |
KTY604 | as YKY213, kre6Δ::kanMX4 | This study |
KTY626 | as KTY604, ura3-52::(KRE6promoter-KRE6-3HA URA3) | This study |
KTY628 | as KTY604, ura3-52::(KRE6promoter-KRE6Δ137-3HA URA3) | This study |
KTY632 | as KTY604, ura3-52::(KRE6promoter-KRE6Δ230-3HA URA3) | This study |
KTY630 | as KTY604, ura3-52::(KRE6promoter-KRE6Δ248-3HA URA3) | This study |
NCYC232 | K1 killer strain | ATCC60782 |