Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Dec 9;286(9):7439–7456. doi: 10.1074/jbc.M110.184382

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 2. — Modulation of B-MYB·STRAP interaction by TGF-β and p53 signals. HEK293 cells transfected with GST-STRAP and FLAG-B-MYB were incubated with or without the following stimuli: TGF-β1 (100 ng/ml, 20 h), 5-fluorouracil (0.38 mm, 30 h), or doxorubicin (Dox, 100 ng/ml, 24 h). Cell lysates were purified on glutathione-Sepharose beads (GST Purification), followed by immunoblotting with an anti-FLAG antibody to determine the association between B-MYB and STRAP (left). HEK293 cell lysates treated with or without the indicated stimuli were immunoprecipitated with an anti-STRAP antibody (IP: α-STRAP) and immunoblotted with an anti-B-MYB antibody to determine the endogenous association between B-MYB and STRAP (right).