FIGURE 3.

Domains of B-MYB involved in STRAP binding. A, the schematic structures of wild-type and deletion mutants of B-MYB are indicated. Numbers indicate the amino acid residues corresponding to the domain boundaries. B, HEK293 cells transfected with the indicated expression vectors were lysed, precipitated using glutathione-Sepharose beads (GST purification), and then immunoblotted with an anti-FLAG antibody to determine the level of B-MYB·STRAP complex formation (top panels). The same blots were re-probed with anti-GST antibody to confirm the expression of GST fusion proteins in the coprecipitates (middle panels).