Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Dec 9;286(9):7439–7456. doi: 10.1074/jbc.M110.184382

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 6. — Modulation of the activated type I TGF-β receptor·SMAD complex by B-MYB. HEK293 cells were transfected with the indicated combinations of plasmid vectors expressing GST-tagged TβR1(TD), an activated type I TGF-β receptor, FLAG-SMAD3, FLAG-SMAD7, and FLAG-tagged wild-type B-MYB or its deletion mutants (DBD, 373/468, TA, and R1), and the cell lysates were subjected to precipitation with glutathione-Sepharose beads (GST purification). Complex formation between TβR1(TD) and SMAD3 (A, left and middle, top panels) or TβR1(TD) and SMAD7 (B, left and middle, top panels) was determined by anti-FLAG antibody immunoblot. The expression level of GST-TβR1(TD) in GST precipitates was determined by anti-GST antibody immunoblot (A and B, left and middle, second panels). HEK293 cells were transfected with the indicated siRNA duplexes (B-MYB siRNAs (#1 and #2) or control scrambled siRNA (Sc)), together with plasmid vectors expressing GST-TβR1(TD), FLAG-SMAD3, or FLAG-SMAD7, and complex formation between TβR1(TD) and SMAD3 (A, right) or TβR1(TD) and SMAD7 (B, right) was determined by anti-FLAG antibody immunoblot (IB). The knockdown of endogenous B-MYB was determined by anti-B-MYB immunoblotting (A and B, right, bottom panels).