FIGURE 1.

Khc-73 domain analysis and construct design. A, sequences of D. melanogaster Khc-73, C. elegans Unc104, and M. musculus KIF1A were aligned using the ClustalW multiple sequence alignment tool at EMBL-EBI. Dark green shaded regions indicate sequence identity, whereas light green shaded regions indicate sequence similarity. B, D. melanogaster Khc-73 sequence was analyzed for coil-forming probability by the COILS program. Probabilities shown are the output using windows of 21 amino acids. C, schematic of Khc-73 constructs used in this work is shown. Label numbers refer to the Khc-73 amino acid boundaries of each construct.