FIGURE 5.

RNAi-mediated knockdown of endogenous Khc-73 identifies Rab5 vesicle-targeting domain in Khc-73. Drosophila BG2 cells were subjected to 7-day RNAi treatment with an RNAi construct targeting the 3′-UTR of Khc-73 or mock treated. On day 4, cells were transfected with constructs containing the mCherry-tagged portion of Khc-73 shown under the control of the metallothionein promoter and GFP-tagged Rab5 under the control of the actin promoter. On day 6, cells were incubated with 50 mm copper sulfate for 16 h. On day 7, time-lapse imaging was performed.