TABLE 1.

Motile and hydrodynamic behavior of Khc-73 motor-containing constructs

Khc-73 construct	Microtubule gliding velocitya	Single molecule motilityb			Sedimentation coefficient	Stokes radius	Molecular mass
		Velocityc	Run lengthc	Frequency			Calculatedd	Predictede
	μm/s	μm/s	μm	events/axoneme/min	×10−13s	Å	kDa	kDa
1–387-GFP	1.45 ± 0.07	1.57 ± 0.26	0.45 ± 0.09	0.09	3.0	42.5	84.2	70.8
1–432-GFP	1.51 ± 0.02	1.58 ± 0.12	1.30 ± 0.49	0.15	3.0	43.3	85.7	76.1
1–547-GFP	1.62 ± 0.10	1.33 ± 0.23	0.76 ± 0.11	0.13	3.0	44.0	87.1	88.8
1–623-GFP	1.58 ± 0.04	1.54 ± 0.46	1.04 ± 0.11	0.28	3.0	45.5	90.1	97.8
1–387-LZ-GFP	N.D.	1.56 ± 0.15	1.51 ± 0.06	6.77	5.1	43.3	144.8	75.0

^a Mean ± S.D. shown for >50 measurements.

^b Each data set comes from multiple experiments using protein from a single preparation.

^c Mean ± S.D. shown for >40 measurements.

^d Molecular mass based on the calculation described under “Experimental Procedures.”

^e Molecular mass based on the sum of amino acids in the Khc-73 construct, linker, GFP, and His₆ affinity tag.