Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Dec 15;286(9):7468–7478. doi: 10.1074/jbc.M110.179242

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 3. — βMHC-Cre;FoxO1^fl/fl/FoxO3^fl/fl mice have increased ischemic injury. A, Western blot assessment of total protein expression of both FoxO1 and FoxO3 in control (FoxO1^fl/flFoxO3^fl/fl) and cardiomyocyte-specific FoxO1- and FoxO3-deficient (βMHC-Cre;FoxO1^fl/fl/FoxO3^fl/fl) mouse hearts. B and C, quantitative representation of A showing 70% reduction of both FoxO1 and FoxO3 in βMHC-Cre;FoxO1^fl/fl/FoxO3^fl/fl mouse hearts compared with FoxO1^fl/fl/FoxO3^fl/fl controls. D and E, NTG, βMHC-Cre, FoxO1^fl/fl/FoxO3^fl/fl, and βMHC-Cre;FoxO1^fl/fl/FoxO3^fl/fl mouse hearts were subjected to 1 h of ischemia followed by 24 h of reperfusion and were stained with triphenyltetrazolium chloride and Evan's blue dye to determine cell viability and the area at risk. Quantification of infarct area (IA) versus AAR shows significant increase in percentage of I/R injury with no significant changes in AAR in βMHC-Cre;FoxO1^fl/fl/FoxO3^fl/fl compared with NTG, βMHC-Cre, and FoxO1^fl/fl/FoxO3^fl/fl mouse hearts. Significance was determined by Student's t test (*, p < 0.05; for NTG, n = 6, βMHC-Cre, n = 7, FoxO1^fl/fl/FoxO3^fl/fl, n = 7, and for βMHC-Cre;FoxO1^fl/fl/FoxO3^fl/fl, n = 8).