FIGURE 2.
Conversion of human PrPC induced by CWD PrPSc after PMCA strain adaptation. A, six separate samples of a 1000-fold dilution of CWD brain homogenate (inoculum A) were used to convert cervid PrPC in three successive rounds of 144 PMCA cycles. Protease-resistant deer PrPSc was measured by Western blotting using antibody 6D11. B, samples from the amplified material were used to attempt conversion of human PrPC by PMCA, and the protease-resistant signal was checked using the human-specific antibody 3F4. NBH No PK, normal brain homogenate used as a control.