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Expression of CD151 constructs in A431 cells. A, schematic representation of CD151 mutant proteins. All constructs had N-DYKDDDDK-C (FLAG) sequences attached to their N termini. B, A431 cells were lysed in a 50/50 mixture of 1% Brij 99 and Brij 96V lysis buffer. Cell lysates were immunoprecipitated (IP) with anti-FLAG M2 agarose and blotted with a polyclonal antibody against the FLAG tag. The mock IP was performed with lysis buffer only instead of cell lysate.
