FIGURE 2.

CD151 mutants display normal cellular localization and expression. A–E, cells grown on glass coverslips overnight were formalin fixed and incubated with CD151 mAb 5C11 followed by Alexa Fluor 488 secondary antibody. F–L, A431 cells grown overnight on glass coverslips were formalin fixed, permeabilized with 0.2% Brij 99 (Sigma-Aldrich), and then incubated with polyclonal anti-FLAG tag antibody followed by Alexa Fluor 488 secondary antibody. Arrowheads in K and L indicate staining of filopodia or retraction fibers indicative of plasma membrane localization.