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PSG1 binds to syndecans 1–4 and glypican-1. A, Namalwa and Namalwa cells stably transfected with syndecans-1, -2, -3, -4, or glypican-1 were incubated with 1 μg of FITC-labeled anti-heparan sulfate 10E4 antibody (Anti-HS Ab) or FITC-labeled isotype matched (IM-Ab) as the control. B, the cells shown in A were incubated with 30 μg/ml PSG1-Fc or the control protein FLAGFc followed by PE-conjugated anti-human Fcγ. The median fluorescence intensity (MFI) of each treatment is shown.
