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Downstream targets of MAPK are affected by PRL/PRL-RS but not the upstream kinases in GG-CL cells expressing PRL-RS. GG-CL cells were transfected with PRL-RS and treated with PRL as indicated. Total protein extracts were analyzed for activated ERK1/2 (A) or activated p38 MAPK pathway (B) by Western analysis using phospho-specific antibodies to ELK1, ATF2, MEK1/2, and MKK3/6. Total ERK1/2 and total p38 were used as loading controls.
