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. 2011 Jan 3;286(9):7609–7618. doi: 10.1074/jbc.M110.166603

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — DUPD1 is a phosphoprotein. A, GG-CL cells were transfected with PRL-RS and treated with PRL for different time points. Total cell lysates were immunoprecipitated with anti-phosphotyrosine antibody or control IgG and immunoblotted for DUPD1. B, GG-CL cells were transfected with PRL-RS and treated with PRL in the presence or absence of AG490. Total cell lysates were immunoprecipitated with anti-phophotyrosine antibody and immunoblotted for DUPD1. Density of DUPD1 was plotted against IgG. C, GG-CL cells were transfected with PRL-RS and treated with PRL. Total cell lysates were immunoprecipitated with DUPD1 and immunoblotted with an anti-phospho-RXRXX(S/T) antibody. The density of phospho-DUPD1 was plotted against IgG. *, p < 0.05 versus 0 min.