FIGURE 1.
p30 expression enhances cell survival under conditions of genotoxic stress. Cell survival was tested in 293T and Jurkat T cells transduced with lentivirus vectors to stably express p30 or mock (GFP vector) prior to exposure to 10-Gy irradiation. Cell survival was monitored using CellTiter 96 AQueous One solution reagent according to the manufacturer's instructions. Data points represent the mean absorbance values of six independent trials plotted over time. Statistical differences were compared using analysis of variance. In 293T cells, statistically significant (asterisk) higher cell survival was noted in the presence of p30 compared with the control (GFP) at day 3 (p ≤ 0.0004), at the time the cells reached confluence. In Jurkat T cells, statistically significant (asterisk) higher cell survival was observed in the presence of p30 compared with the control (GFP) at days 3, 4, and 5 (p ≤ 0.0044, 0.0003, and 0.0185, respectively).