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. 2010 Dec 30;286(9):7681–7691. doi: 10.1074/jbc.M110.188532

FIGURE 1.

FIGURE 1.

Effect of PC4 knockdown and overexpression on the LHR promoter activity. A, left, whole cell lysates of MCF-7 cells transfected with two specific siRNAs (PC4–1, PC4–2) against PC4 or negative control scrambled siRNA (NTC) were analyzed for expression of PC4 by Western blot with PC4 antibody. The expression of actin served as control. Right, reporter gene analyses of LHR promoter activity in MCF-7 cells transfected with PC4–1, PC4–2 siRNA, and NTC siRNA. Relative luciferase activity was expressed as the percentage over the basal promoter activity in cells transfected with NTC siRNA, which was set as 100. B, LHR promoter was cotransfected with pcDNA3.1 vector, pCMV-Sp1, pcDNA 1.1-Sp3 and pCMV-PC4 expression vectors, or both Sp1/PC4, Sp3/PC4 vectors in MCF-7 cells followed by luciferase reporter gene assay. The amount of transfected DNA in each well was equalized by addition of empty vector (pcDNA3.1). Relative luciferase activity was expressed as fold-induction over the promoter activity with transfection of empty vector only.