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. 2010 Dec 30;286(9):7681–7691. doi: 10.1074/jbc.M110.188532

FIGURE 7.

FIGURE 7.

Effect of PC4 knockdown on TSA-mediated recruitment of Pol II, TFIIB, and other promoter-associated factors at LHR promoter. A and B, quantitative ChIP analyses of the association of Sp1, PolII, TFIIB, p107, HDAC1, mSin3A, HDAC2, and PP1 with the LHR promoter in NTC and PC4 siRNA-transfected cells. Cells were treated with or without TSA (500 ng/ml) for 16 h. C, proposed role of PC4 in TSA-induced LHR activation. TSA-induced LHR activation is achieved by the combined actions of chromatin changes, release of inhibitory factors (p107, HDAC1/2/mSin3A, and phosphatase PP1), and recruitment of PC4, which is independent to PI3K/PKCζ/Sp1 phosphorylation. PC4 might function as a linker to bridge Sp1 and one or more components of the PIC.