FIG. 2.—

Import properties of heterologously expressed haptophytic sequences. The topogenic signals of sDer1-1 and sUba1 direct GFP to the PPC. The GFP fusion comprising the targeting signal of sCdc48 predominantly localizes to the ER. The presequences of BiP and AtpG lead to either ER or stromal localization of GFP, respectively. The host versions of Uba1 and Cdc48 localize within the cytosol. Columns from left to right: light microscopic images, chlorophyll autofluorescence, GFP fluorescence and YFP fluorescence, respectively, merged chlorophyll and GFP/YFP fluorescence. Scale bar represents 10 μm.