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. 2011 Jan 31;11:13. doi: 10.1186/1472-6750-11-13

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Copyright ©2011 Leyva et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Flow cytometry analysis of human blood monocyte-derived macrophages transduced with lentivirus at different MOIs. Cells from donor 2 were transduced with lentivirus VRX494 and VRX1090 two hours after plating using MOIs of 5 (A), 10 (B), 25 (C), and 50 (D) at a final concentration of 3, 6, 15, and 30 × 10⁶transducing units/ml, respectively. Each bracketed region in the histogram corresponds to % EGFP-positive cells and shaded area corresponds to non-transduced cell control. Flow cytometry was performed on day 8 after cell plating.