Table 2.

Transduction efficiency* of human blood monocyte-derived macrophages using different viral systems

	Transduction efficiency	MOI	Viral system	Differentiation Method	Quantification technique
Haddada H, et al. [13]	40-80%	100	Adenovirus	HS	Beta-gal activity
Huang S, et al. [30]	52%	1000	Adenovirus	M-CSF+FBS	Beta-gal activity
Schneider SD, et al. [33]	20%	100	Adenovirus	HS	LacZ activity
De S, et al. [31]	10%	100	Adenovirus	GM-CSF+FBS	Beta-gal activity
Foxwell B, et al. [12]	> 90%	100	Adenovirus	M-CSF+FBS	Beta-gal activity flow cytometry
Heider H, et al. [14]	80%	30	Adenovirus	HS	GFP fluorescence microscopy
White S, et al. [34]	15%	1	HIV/SIV based lentivirus	GM-CSF+HS	GFP fluorescence microscopy
Schroers R, et al. [20]	30%	100	HIV based lentivirus	GM-CSF+FBS	YFP flow cytometry
Neil S, et al. [19]	20%	10	HIV based lentivirus	HS	GFP flow cytometry
Ravot E, et al. [32]	40%	0.65	HIV based lentivirus	HS+FBS	GFP flow cytometry
Lu Y, et al. [21]	40%	50	HIV based lentivirus	HS+FBS	GFP fluorescence microscopy
Jarrosson-Wuilleme L, et al. [28]	30%	25	HIV based lentivirus	GM-CSF+FBS	GFP flow cytometry
Zeng L, et al. [29]	50%	100	HIV based lentivirus	HS+FBS	GFP fluorescence microscopy
Current report	80-90%	10	HIV based lentivirus	M-CSF+FBS	EGFP flow cytometry

* Percentage transduced cells, values correspond to the highest value listed in the respective manuscript. MOI = multiplicity of infection, HS = human serum, GM-CSF = granulocyte-macrophage colony-stimulating factor, M-CSF = macrophage colony-stimulating factor, FBS = fetal bovine serum