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. 2011 Feb 25;6(2):e16799. doi: 10.1371/journal.pone.0016799

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A–D) Cuticle preparations of dead embryos. (A and B) Homozygous or transheterozygous combinations of the ico alleles II032, G^A1, G^A25, and B^A18 die as embryos with head involution defects. (C and D) Animals heterozygous for ico EMS-mutant alleles are embryonic lethal in combination with dpp^hr4, indicating that they exhibit reduced DPP signaling.