. 2011 Feb 25;6(2):e16799. doi: 10.1371/journal.pone.0016799

Table 1. Rescue of ico mutants by fly and human EF-G1 proteins.

	Adults	Rescue
UAS-CG4567/w; ico *^GA1* ico *^{Del EY2}; +/arm+da-GAL4* UAS-CG4567/w; CyO/ico *^{Del EY2}; +/arm+da-GAL4*	6770	67/70 = 96%
UAS-CG4567/w; ico *^{Del EY1}* /ico *^{Del EY2}; +/arm+da-GAL4* UAS-CG4567/w; CyO/ico *^{Del EY2}; +/arm+da-GAL4*	7270	72/70 = 103%
UAS-TP-GFP-CG4567/w; ico *^{Del EY1}* /ico *^{Del EY2}; +/arm+da-GAL4* UAS-TP-GFP-CG4567/w; CyO/ico *^{Del EY2}; +/arm+da-GAL4*	6570	65/70 = 93%
UAS-CG4567-G538E/w; ico *^{Del EY1}* /ico *^{Del EY2}; +/arm+da-GAL4* UAS-CG4567-G538E/w; CyO/ico *^{Del EY2}; +/arm+da-GAL4*	0102	0/102 = 0%
UAS-CG4567-ΔCstop/w; ico *^{Del EY1}* /ico *^{Del EY2}; +/arm+da-GAL4* UAS-CG4567-ΔCstop/w; CyO/ico *^{Del EY2}; +/arm+da-GAL4*	6166	61/66 = 92%
UAS-hEF-G1/w; ico *^{Del EY1}* /ico *^{Del EY2}; +/arm+da-GAL4* UAS-hEF-G1/w; CyO/ico *^{Del EY2}; +/arm+da-GAL4*	6773	67/73 = 92%
UAS-hEF-G1-N174S/w; ico *^{Del EY1}* /ico *^{Del EY2}; +/arm+da-GAL4* UAS-hEF-G1-N174S/w; CyO/ico *^{Del EY2}; +/arm+da-GAL4*	7492	74/92 = 80%
w/w; ico *^{Del EY1}* /ico *^{Del EY2}; UAS-hEF-G1-S321P/arm+da-GAL4* w/w; CyO-TM6B/ico *^{Del EY2}; arm+da-GAL4*	3895	38/95 = 40%
UAS-CG31159/w; ico *^{Del EY1}* /ico *^{Del EY2}; +/arm+da-GAL4* UAS-CG31159/w; CyO/ico *^{Del EY2}; +/arm+da-GAL4*	0111	0/111 = 0%
w/w; ico *^{Del EY1}* /ico *^{Del EY2}; UAS-CG31159 (2x)/arm+da-GAL4* w/w; ico *^{Del EY2}; CyO-TM6B/arm+da-GAL4*	097	0/97 = 0%

Females with ico mutations and fly or human transgenes on the X or third chromosome (UAS-EF-G1/UAS-EF-G1; ico/CyO; +/+ or w/w; ico; UAS-EF-G1/CyO-TM6B) were crossed to males with ico mutations and the ubiquitous GAL4 drivers armadillo-GAL4 and daughterless-GAL4 balanced over chromosomes with dominant curly wing and tubby body markers (w/Y; ico ^{Del EY2}; arm+da-GAL4/CyO-TM6B). The numbers of the rescued homozygous ico mutant females, which have straight wings and are not tubby, were compared to their heterozygous ico sisters, which have curly wings. (Row 3) TP-GFP-CG4567 transgenes that express a GFP-tagged Drosophila EF-G1 protein inserted after the N-terminal target sequence (TP) encode a functional protein that can rescue ico mutants. (Row 4, 9, and 10) Transgenes that express the II032 mutant EF-G1 or Drosophila EF-G2 (CG31159) proteins do not rescue ico mutants. (Row 5) CG4567-ΔCstop is a transgene with a premature stop codon that lacks the C-terminal tail. (Row 7 and 8) The adults rescued with hEF-G1-N174N and hEF-G1-S321P hatched at least 3-5 days later than their siblings did. (Row 8 and10) The number of the animals rescued with the hEF-G1-S321P or CG31159 transgenes was compared to curly and tubby animals with the darker eye color of the arm+da-GAL4.