Table 1. Primer sequences used for QPCR.

Name	Type	Sequence (5′-3′)	Expected length (bp)	Optimal annealing temperature (°C)
TLR1	Forward	CAGCAGCCTCAAGCATGTCTA	82	60
	Reverse	CAGCCCTAAGACAACAATACAATAGAAGA
TLR2	Forward	CTCCTGTGAACTCCTGTCCTT	74	60
	Reverse	AGCTGTCTGGCCAGTCAAC
TLR3	Forward	GATTGGCAAGTTATTCGTC	205	54
	Reverse	GCGGAGGCTGTTGTAGG
TLR4	Forward	GATTGCTCAGACATGGCAGTTTC	135	54
	Reverse	CACTCGAGGTAGGTGTTTCTGCTAA
TLR5	Forward	GGGCAGCAGAAAGACGGTAT	61	60
	Reverse	CAGGCACCAGCCATCCTTAA
TLR6	Forward	GGAGCCTTCAGTAGCCTTTC	115	60
	Reverse	GGTTGTTCCCCGCTGTTAT
TLR7	Forward	GTTTTACGTCTACACAGTAACTCTCTTCA	75	60
	Reverse	TTCCTGGAGGTTGCTCATGTTTT
TLR8	Forward	GGGGTAACACACCGTCTA	150	60
	Reverse	GTCAAGGCGATTTCCACT
TLR9	Forward	CCGAAGACCTAGCCAACCT	70	60
	Reverse	TGATCACAGCGACGGCAATT
IFN-α	Forward	GCAACCCTCCTAGACTCATTCTG	83	65
	Reverse	ACCCCTACCTGCTGCATCAG
IFN-β	Forward	CTTGGGTGACATCCACGACTAC	92	60
	Reverse	GGCATAGCTGTTGTACTTCTTGTCTT
IFN-γ	Forward	AGAGCCTCCTCTTGGATATCT	309	65
	Reverse	GCTTCCTTAGGCTAGATTCTGGTG
IL-1β	Forward	CTGTGACTCGTGGGATGATGAC	322	53
	Reverse	CTTCTTCTTTGGGTATTGTTTGG
IL-12p35	Forward	AAGACATCACACGGGACAAA	300	60
	Reverse	GATTCAGAGACCGCATTAGC
IL-6	Forward	AAGAAAGACAAAGCCAGAGTC	263	57
	Reverse	CACAAACTGATATGCTTAGGC
IL-4	Forward	TGCACCGAGATGTTTGTACCAGA	92	63
	Reverse	TTGCGAAGCACCCTGGAAG
TGF-β	Forward	GGACTACTACGCCAAAGAAG	294	57
	Reverse	TCAAAAGACAGCCACTCAGG
GAPDH	Forward	CGGCAAGTTCAACGGCACAG	148	60
	Reverse	GAAGACGCCAGTAGACTCCACGAC