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. 2011 Feb 25;6(2):e17270. doi: 10.1371/journal.pone.0017270

Figure 4. Effect of Nse1 on the interaction between S.pombe Nse3 and Nse4.

Figure 4

(A) Yeast-2-hybrid plasmids expressing Nse3, either wild-type or mutated as indicated, fused to the Gal4 DNA-binding domain, and wild-type Nse4 fused to the Gal4 activation domain, were co-expressed with either empty vector (v) or Nse1 (1) in yeast cells, which were subsequently plated in the indicated media and grown at 30°C. AT, 3-aminotriazole. (B) Spot tests of Nse3 wild-type cells (wt:YL), Y264A/L265A (AA), L265A (YA) and Y264A (AL) plated under the indicated conditions.