Table 1. Interaction of S.pombe Nse3 mutants with Nse1 and Nse4.
| Mutation | Nse1 | Nse4 | Phenotype | Mutation | Nse1 | Nse4 | Phenotype |
| WT | + | + | WT | M214A | + | − | |
| L93A | + | + | T215A | + | +/− | WT | |
| V94A | − | + | V216A | + | − | ||
| R95A | + | + | I217A | − | − | WT | |
| V98A | − | + | A218G | + | − | ||
| R99A | − | + | WT | F219A | + | − | |
| Y100A | + | + | I220A | − | − | ||
| I102A | − | +/− | V222A | + | − | WT | |
| Q105A | + | + | S223A | + | − | ||
| S107A | + | + | WT | V227A | + | + | |
| H108A | + | + | H229A | + | + | WT | |
| N109A | + | + | L232A | − | − | ||
| T112A | + | + | WT | F235A | + | − | WT |
| R113A | + | + | L236A | + | − | WT | |
| K114A | + | + | E238A | + | + | ||
| K119A | + | + | L239A | + | − | ||
| F121A | − | +/− | L240A | + | − | ||
| E123A | + | + | P247A | + | − | ||
| T125A | + | + | L248A | + | − | ||
| R127A | + | + | H249A | + | − | ||
| F130A | +/− | + | I252A | + | − | ||
| Q131A | + | + | S255A | + | − | WT | |
| V133A | +/− | + | S257A | + | + | WT | |
| F134A | − | + | L259A | + | − | ||
| E135A | + | + | V260A | + | + | ||
| E136A | + | + | R261A | + | + | ||
| A137K | − | + | Q262A | − | − | WT | |
| R139A | +/− | + | Y264A | − | − | WT | |
| Q140A | + | + | L265A | − | − | WT | |
| L141A | − | +/− | WT | R267A | + | + | |
| S144A | + | + | WT | F276A | − | + | WT |
| F145A | + | + | WT | Y278A | − | − | |
| G146A | − | +/− | Y279A | + | + | ||
| F147A | − | + | E287A | + | + | WT | |
| L149A | − | + | L293A | − | − | ||
| V152A | + | + | F296A | + | − | ||
| S155A | + | + | V297A | + | − | ||
| H180A | + | + | F300A | + | − | ||
| Y182A | + | + | F301A | + | + | ||
| V184A | + | + | SUMMARY | Structural | 13 | ||
| L185A | − | + | Nse4-specific | 20 | |||
| T188A | + | + | Nse1-specific | 12 | |||
| L199A | + | + | No disruption | 37 | |||
| F212A | − | − | Total | 82 | |||
Combinations of the indicated mutant Nse3 proteins with Nse1 or Nse4 were analysed in the Y2H system. + and − signify whether or not an interaction was detected. Some of the mutations were introduced into the S. pombe genome and sensitivity to MMS and HU was analysed. WT indicates no sensitivity.