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Mutants (Basal mutant, P107C/, N111C/, E122C/, A124C/, L125C/, and L128C/WT) were purified from COS-1 cells after regeneration with 25 µM 11-cis-retinal. UV-vis spectra were recorded in the dark, after acidification, and after illuminating the samples for 30 sec at 20°C.
