Table 2. Comparison of the reactivity of cysteine mutant T108C-R135C/Basal mutant at Helix-III of rhodopsin with 4-PDS after photoactivation.
mutant | Rate (min−1)a | mutant | Rate (min−1)a | mutant | Rate (min−1)a |
T108C | >1.4b | T118C | 0.054±0.006 | V129C | 0.057±0.006 |
G109C | >1.4b | L119C | 0.074±0.008 | V130C | 0.053±0.011 |
L112C | 0.242±0.0537 | G120C | 0.032±0.005 | L131C | >1.4b |
E113C | >1.4b | G121C | 0.302±0.06 | A132C | 0.177±0.035 |
F115C | 0.088±0.011 | E122C | 0.036±0.006 | I133C | 0.021±0.003 |
F116C | 0.047±0.008 | I123C | 0.048±0.007 | R135C | 0.103±0.013 |
A117C | 0.048±0.007 | S127C | 0.042±0.006 | Basal mutant | 0.041±0.005 |
The error given of each value of the pseudo-first-order rate constant is the standard deviation.
These reactions were complete at the first time point taken (∼0.5 min).