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Organization of the tqa gene cluster and genetic verification of involvement in 1 biosynthesis. (A) The tqa gene cluster; (B) Knockout strategy used to inactivate tqaA; (C)HPLC (280 nm) traces of metabolic extracts from single gene deletion strains of P. aethiopicum. Trace i: wild type strain producing 1, 11–13; trace: ii, ΔtqaA; trace iii, ΔgsfA. This strain was constructed to eliminate the high titer metabolites 12 and 13; and trace iv, ΔgsfA/ΔtqaK. ♦ indicates repeating sequences.
