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. Author manuscript; available in PMC: 2012 Mar 30.
Published in final edited form as: Mol Cell Endocrinol. 2011 Jan 14;335(2):148–157. doi: 10.1016/j.mce.2011.01.004

Table 1.

A.PIII deletion primers Primer sequence (5’ – 3’)
PIII-XhoI forward CGGCTCGAGACTGTTTTGCCTCCAAGCAAGGA
PIII-HindIII reverse ACCAAGCTTTTTTCAGTGACAGGTAAAGCTGTTTCC
-386-PIII forward CGGCTCGAGTAGTGAAGAAAGAGTGAACAAG
-398-PIII forward CGGCTCGAGGACTCCTCCTCTAGTGAAGAA
B. PIII site-directed mutagenesis primers Primer sequence (5’ – 3’)*
AP1 forward reverse CGCCTTCCTGCTCTGTCTAACTAACTCCTCTCCTGCGTTCTGG
CCAGAACGCAGGAGAGGAGTTAGTTAGACAGAGCAGGAAGGCG
Sp1A forward reverse GCATGTTGCAACACTGACTACTCATCTAGTGAAGAAAGAG
CTCTTTCTTCACTAGATGAGTAGTCAGTGTTGCAACATGC
Sp1B forward reverse GAGCTGGGCTTTCCACGCATTCCTGCTCTGTCTCACTC
GTGAGACAGAGCAGGAATGCGTGGAAAGCCCAGCTC
C/EBP forward reverse CCAGCAAGGAACATGCATGCTACAACACTGACTCCTCCTCTAG
CTAGAGGAGGAGTCAGTGTTGTAGCATGCATGTTCCTTGCTGG
Double forward reverse CAGCAAGGAACATGCATGTTACAATACTGACTACTCATCTAG
CTAGATGAGTAGTCAGTATTGTAACATGCATGTTCCTTGCTC
Triple forward reverse CAGCAAGGAACATGCATGTTACAATACTGACTACTCATCTAG
CTAGATGAGTAGTCAGTATTGTAACATGCATGTTCCTTGCTC
* Template for all site-directed mutagenesis constructs is -439-PIII deletion mutant; template for triple had Sp1B site already mutated.
C. Gel shift oligos Primer sequence (5’ – 3’)
Sp1A forward reverse ATGTTGCAACACTGACTCCTCCTCTAGTGAAGA
TCTTCACTAGAGGAGGAGTCAGTGTTGCAACAT
Sp1A mutated forward reverse ATGTTGCAACACTGACTACTCATCTAGTGAAGA
TCTTCACTAGATGAGTAGTCAGTGTTGCAACA
Sp1B forward reverse GAGCTGGGCTTTCCCCGCCTTCCTGCTCTGTCT
AGACAGAGCAGGAAGGCGGGGAAAGCCCAGCTC
Sp1B mutated forward reverse GAGCTGGGCTTTCCACGCATTCCTGCTCTGTCT
AGACAGAGCAGGAATGCGTGGAAAGCCCAGCTC
C/EBP forward reverse AGGAACATGCATGTTGCAACACTGACTCCT
AGGAGTCAGTGTTGCAACATGCATGTTCCTTGC
C/EBP mutated forward reverse GCAAGGAACATGCATGTTACAATACTGACTCCT
AGGAGTCAGTATTGTAACATGCATGTTCCTTGC