. 2011 Feb;14(1):45–56. doi: 10.1089/rej.2010.1085

Table 2.

Baseline Values for Selected Biomarkers, Relevant Blood/Immune Variables, and Relationship to Age

	Age	Lymphocyte telomere length^a	Granulocyte telomere length	CMV IgG Antibody^b	WBC^c(UCLA)	#Lymph (UCLA)	% CD3 (UCLA)	# CD3 (UCLA)	% CD8 (UCLA)	# CD8 (UCLA)	% CD8+ CD28⁻ (UCLA)	# CD8+ CD28⁻ (UCLA)
Mean	62.5	5.73 kb	6.71	1.83	5690	1850	70.1	1320	22.8	435	34.7	175
SD	12.5	1.05 kb	0.88	1.77	1520	565	8.57	479	9.14	250	19.9	180
n	114	114	114	88	113	113	113	113	113	113	113	113
m^d	NA	−55 bp	−34 bp	0.024	22	−6.6	−0.14	−6.4	−0.08	−3.0	0.37	1.6
R squared	NA	0.42	0.24	0.03	0.032	0.021	0.044	0.029	0.012	0.023	0.055	0.190
p value	NA	4.8E-15	3.1E-8	0.11	0.057	0.12	0.026	0.073	0.24	0.11	0.012	0.25

	# CD28⁺ (calc)^e	% CD28⁺(calc)	# CD95⁻ (UCLA)	% CD95⁻ (UCLA)	# Lymphocytes	% Lymphocytes	# NK	% NK	% CD19	# CD19	% CD3	# CD3
Mean	260	65	63	18	1500	28	221	15.0	11.7	186	71.7	1150
SD	132	20	53	12	524	8.70	100	7.2	5.04	100	8.16	414
n	113	113	57	57	54	103	103	103	103	103	103	103
m	−4.6	−0.37	−2.7	−0.51	−2.5	−0.21	2.2	0.21	−0.13	−2.6	−0.091	−3.8
R squared	0.19	0.055	0.47	0.44	0.004	0.09	0.07	0.13	0.04	0.1	0.014	0.00
p value	1.4E-06	0.012	6.7E-9	6.4E-6	0.72	0.002	0.006	0.00015	0.00067	0.0011	0.17	0.25

	CD4/CD8 ratio	% CD4⁺	% CD8⁺	# CD8⁺	# WBC	# Neutrophils	% Neutrophils	Glucose	Serum insulin	DHEA sulfate	Testosterone (free)	Testosterone (total)
Mean	2.8	49	22	370	5700	3600	61	97	7.1	148	33	400
SD	2.7	8.9	8.3	200	1600	1300	9.3	15	7.0	131	42	340
n	103	103	103	103	106	102	103	107	107	106	106	106
m	0.064	0.045	-0.10	-2.1	230	31	0.17	0.33	0.025	−2.6	0.23	1.1
R squared	0.007	0.004	0.023	0.017		0.08	0.05	0.08	0.002	0.06	0.005	0.002
p value	0.0024	0.53	0.13	0.19	0.051	0.039	0.020	0.00460	0.64	0.011	0.47	0.31

	Cholesterol (total)	Cholesterol (LDL)	SBP	DBP	Homocysteine	Serum folate	Vitamin D 25-OH total	Vitamin B12
Mean	190	110	130	77	11	17	40	790
SD	38	34	19	10	3.6	5.8	16	410
n	105	104	97	97	107	103	107	105
m	−0.29	−0.37	0.51	−0.018	0.066	−0.12	0.11	6.1
R squared	0.01	0.02	0.108	0.000	0.05	0.02	0.007	0.036
p value	0.32	0.16	0.0010	0.83	0.016	0.0086	0.38	0.053

^{^a}

Lymphocyte and granulocyte telomere lengths are from FlowFISH analyses.

^{^b}

For some subjects, CMV antibody titers were first taken at a postbaseline time point.

^{^c}

Replicate blood samples were collected and sent to UCLA Clinical Labs for analyses which focused on assessment of senescent CD28⁻/CD8⁺ T cells, and to Quest Diagnostics for routine blood counts. Discrepancies in numbers for the same lab analysis likely reflect variations between laboratory techniques. Table entries represent data from Quest except where UCLA is indicated.

^{^d}

Slope of the linear regression line of variable indicated versus age of donor.

^{^e}

Number and percent CD8⁺CD28⁺ cells were derived from the measured number of CD8⁺CD28⁻ and total CD8⁺ cells.

CMV, Cytomegalovirus; IgG, immunoglobulin G; SD, standard deviation; NK, natural killer; WBC, white blood cells; DHEA, 5-dehydroepiandrosterone; LDL, low-density lipoprotein; SBP, systolic blood pressure; DBP, diastolic blood pressure; FlowFISH, Flow fluorescence in situ hybridization.