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Autoradiogram of a polyacrylamide gel showing TdT insertion of xDNA and DNA nucleoside triphosphates after 1 h. Lane 1: incorporation of dTTP, 10 µM; lane 2: dGTP, 10 µM; lane 3: dCTP, 10 µM; lane 4: dATP, 10 µM; lane 5: dxTTP, 10 µM; lane 6: dxGTP, 10 µM; lane 7: dxCTP, 10 µM; lane 8: dxATP, 10 µM. M: 10 bp size marker; P: unreacted DNA primer (sequence 5′-dATACCAAAGT-3′).
