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Spectra of xDNTP monomers and of TdT reaction products partially purified by size exclusion. (A) Normalized emission spectra of TdT reaction products with dxATP (green) and monomer dxATP (purple) in buffer (excitation 333 nm). (B) Normalized emission spectrum of the TdT reaction product with dxCTP (blue) and monomer dxCTP (red) in PIPES buffer (excitation 330 nm). Spectra were measured in PIPES buffer (100 mM NaCl, 10 mM MgCl2 and 10 mM Na•PIPES, pH 7.0).
