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Fluorescence microscopy images of TdT-mediated xDNA reaction products on CPG with (dT)25 primer attached. (A) [dxATP] = 68 µM, TdT omitted. (B) [dxATP] = 68 µM, [TdT] = 1 U/µl. (C) [dxCTP] = 140 µM, TdT omitted. (D) [dxCTP] = 140 µM, [TdT] = 1 U/µl. Excitation 330–380 nm for all images; emission was measured with a 420 nm long-pass filter for (A), (B) and 400 nm long-pass filter for (C and D).
